Publications
- Kime, MJ (1984) FEBS Letts. 175, 259-262. Title:
"Assignment of resonances of exchangeable protons in
the NMR spectrum of the complex formed by E.coli
ribosomal protein L25 and uniformly nitrogen-15 enriched
5S RNA fragment" [Readers will note that this was
part of the pioneering work on NMR spectroscopic studies
of protein-nucleic acid interactions on solution
samples.]
- Kime, MJ (1984) FEBS Letts. 173, 342-346. Title:
"Assignment of resonances in the E.coli 5S
RNA fragment proton NMR spectrum using uniform
nitrogen-15 enrichment" [Readers with specialist
scientific knowledge may note this use of uniform
nitrogen-15 enrichment in NMR studies of
biomacromolecules, and, in particular, for the
exploitation of the heteronuclear spin-coupling between
nitrogen-15 and protons. It is interesting to observe
that many scientists have subsequently devoted themselves
(in worthwhile experiments) to exploiting heteronuclear
spin-coupling between 1/2 spin nuclei and protons (and
other nuclei) of biomacromolecules (and polypeptides)
with uniform isotopic enrichment of the (not proton) 1/2
spin heteronuclear isotope. Previously, interested
scientists with the resources and the expertise had
sought to selectively-enrich biomacromolecules with 1/2
spin heteronuclear isotopes at particular positions in
molecules rather than to enrich uniformly.]
- Kime, MJ, Gewirth, DT, Moore, PB (1984)
Biochemistry 23, 3559-3568 [Readers with specialist legal
(jurisprudential) knowledge will recognise the surname
"Gewirth". I understand my co-worker was the
son of the professor of jurisprudence associated with
what I recollect (I wonder how accurately) was known as
the "American Realism" school of
jurisprudence.]
- Kime, MJ, Moore, PB (1984) Biochemistry 23,
1688-1695. Title: "The E.coli 5S RNA-protein
L25 nucleoprotein comples: Effects of RNA binding on the
protein structure and the nature of the
interaction."
- Moore, PB, Kime, MJ, Leontis NB, Abdel-Meguid, SS
(1983) Journal Biomolecular Structure and Dynamics 1,
383-394
- Mann, S, Kime, MJ, Ratcliffe, RG, Williams, RJP
(1983) Journal Chemical Society, Dalton Transactions
771-774. Title: "Precipitation within Unilamellar
Vesicles. Part 2. Membrane Control of Ion
Transport."
- Kime, MJ, Moore, PB (1983) Biochemistry 22,
2615-2622
- Kime, MJ, Moore, PB (1983) Biochemistry 22,
2622-2629. Title: "Nuclear Overhauser Experiments at
500 MHz on the Downfield Proton Spectra of 5S RNA and its
Complex with Ribosomal Protein L25"
- Kime, MJ, Moore, PB (1983) Federation of European
Biochemical Societies Letters 153, 199-203 [Readers will
note the quality of the high-field Fourier transform
proton NMR spectra of (what were) biomacromolecules (not
mere polypeptides or other relatively small molecules) in
aqueous solution using the simple "twin-pulse"
radiofrequency irradiation pulse sequence rather than the
more elaborate and more demanding "Redfield pulse
sequence" (which appears to have been so demanding
that I do not believe I worked on any spectrometer to
have performed it satisfactorily).]
- Kime, MJ, Moore, PB (1982) Nucleic Acids Research
10, 4973-4983
- Kime, MJ, Ratcliffe, RG, Williams, RJP, Loughman,
BC (1982) J. Experimental Botany 33, 656-669
- Kime, MJ, Ratcliffe, RG, Loughman, BC (1982) J.
Exp. Bot. 33, 670-681. Title: "The Application of 31P
NMR to Higher Plant Tissue II. Detection of Intracellular
Changes." [Readers may note that it was in studying
potato tuber tissue (one of the several forms of higher
plant tissue studied) by NMR for this study that it
became apparent that the phytic acid (the chemical form
in which phosphate is stored in potato tuber tissue and,
of course, it has been known for decades if not longer
that this was so but it appears to have been assumed that
it was distributed more or less uniformly throughout the
tuber) was localised in the pith/core tissue of the
potato tuber and in the waxy looking connections
radiating from the pith to the growth points of the
tuber.]
- Kime, MJ, Ratcliffe, RG, Moore, PB, Williams, RJP
(1981) Eur. J. Biochem. 116, 269-276
- Kime, MJ, Ratcliffe, RG, Moore, PB, Williams, RJP
(1980) European Journal of Biochemistry 110, 493-498.
Title: "On the Renaturation of Ribosomal Protein
L11."
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